SARS-CoV-2 (COVID-19, 2019-nCoV) Spike S2 Domain (Gold 40nm)

Cat# 544496-100ul

Size : 100ul

Brand : US Biological

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Phone : +1 850 650 7790


544496 SARS-CoV-2 (COVID-19, 2019-nCoV) Spike S2 Domain (Gold 40nm)

Clone Type
Polyclonal
Host
chicken
Swiss Prot
P0DTC2
Isotype
IgY
Grade
Affinity Purified
Applications
LF
Accession #
QHD43416
Shipping Temp
Blue Ice
Storage Temp
-20°C

Spike glycoprotein comprises two functional subunits responsible for binding to the host cell receptor (S1 subunit) and fusion of the viral and cellular membranes (S2 subunit). For many coronavirus (CoVs), S is cleaved at the boundary between the S1 and S2 subunits, which remain non-covalently bound in the prefusion conformation. The distal S1 subunit comprises the receptor-binding domain(s) and contributes to stabilization of the prefusion state of the membrane-anchored S2 subunit that contains the fusion machinery. S is further cleaved by host proteases at the so-called S2' site located immediately upstream of the fusion peptide in all CoVs. This cleavage has been proposed to activate the protein for membrane fusion via extensive irreversible conformational changes. However, different CoVs use distinct domains within the S1 subunit to recognize a variety of attachment and entry receptors, depending on the viral species. Endemic human coronaviruses OC43 and HKU1 attach via their S domain A to 5-N-acetyl-9-O-acetyl-sialosides found on glycoproteins and glycolipids at the host cell surface to enable entry into susceptible cells. MERS-CoV S uses domain A to recognize non-acetylated sialoside attachment receptors, which likely promote subsequent binding of domain B to the entry receptor, dipeptidyl-peptidase 4. SARS-CoV and several SARS-related coronaviruses (SARSr-CoV) interact directly with angiotensin-converting enzyme 2 (ACE2) via SB to enter target cells.||Applications: |Suitable for use in Lateral Flow. Other applications not tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Biological Activity:|Can bind with human ACE2 in a functional ELISA assay||Storage and Stability:|May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.

Applications
Product Type: Pab|Isotype: IgY|Host: chicken|Concentration: Not Determined |Form: Supplied as a liquid in PBS, 1% BSA, 0.025% Tween20, 0.02% sodium azide. Labeled with 40nm Gold. OD530nm= 4.0|Purity: Purified by affinity chromatography. |Immunogen: Recombinant protein corresponding to full length SARS-CoV-2 (2019-nCoV) spike protein (S2) domain, expressed in 293 cells (HEK293).|Specificity: Recognizes SARS-CoV Spike protein (S2).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant protein corresponding to full length SARS-CoV-2 (2019-nCoV) spike protein (S2) domain, expressed in 293 cells (HEK293).
Form
Supplied as a liquid in PBS, 1% BSA, 0.025% Tween20, 0.02% sodium azide. Labeled with 40nm Gold. OD530nm= 4.0
Purity
Purified by affinity chromatography.
Specificity
Recognizes SARS-CoV Spike protein (S2).