RFP Western Blot Kit: for RFP Chemiluminescent Western Blotting
Cat# KCA379
Size : 1Each
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for RFP Chemiluminescent Western Blot Kit
Product Details
RFP Western Blot Kit: for RFP Chemiluminescent Western Blotting - KCA379
Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit, RFP, rRFP, mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange, mStrawberry
Polyclonal
Chemiluminescent Western Blot Kit
Target Details
dsRed - View All dsRed Products
RFP, mBanana, mCherry, mOrange, mPlum, mStrawberry, rRFP, tdTomato
Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for RFP combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of recombinant proteins containing wild type Red Fluorescent Protein (DsRed or RFP) and its variants such as mBanana, mCherry, DsRed2, E2-Crimson, mOrange, mOrange2, mPlum, mRaspberry, mStrawberry and tdTomato. The Chemiluminescent Western Blot Kit design includes straightforward procedures and color-coding to allow for ease of use. This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.
Q9U6Y8 - UniProtKB
Application Details
WB
Use Rockland Immunochemicals’ Anti-RFP Chemiluminescent Kit for Western Blotting for detection of RFP-tagged recombinant proteins by western blot. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
Formulation
Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
Shipping & Handling
Wet Ice
See kit insert for complete instructions.
See kit insert for complete instructions.
Background
Red Fluorescent Protein (RFP) Western Blotting kit allows for the detection of RFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-RFP antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
References (2)
Ibayashi M et al. (2023). Lipid droplet synthesis is associated with angiogenesis in mouse ovarian follicles. Biol Reprod.
Applications
WB, IB, PCA PubMed
Boshans, LL et al. (2021). Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2. Scientific Reports
Applications
WB, IB, PCA PubMed
Related Protocols
Adherent Cell Lysis Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This material is subject to proprietary rights and is sold under license from ROCKLAND IMMUNOCHEMICALS Inc. This product is licensed for sale only for 'research-use' only. There is no implied license hereunder for any commercial use. FemtoMax® is a trademark of ROCKLAND IMMUNOCHEMICALS Inc. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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