Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Biotin
Specifications
Product Data
Recommended Dilution
Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions ELISA: 1/150,000. Western blot: 1/2,000-1/20,000. Immunohistochemistry: 1/1,000-1/10,000.
Reactivity
Rat
Antibody Host
Goat
Clonality
Polyclonal
Immunogen
Rat IgG, IgA and IgM whole molecules.
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgG, IgA and IgM. This reagent is suitable for the detection of all rat immunoglobulin subclasses, isotypes and chain combinations.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative Label: Biotin State: Lyophilized purified Ig fraction. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule
Reconstitution Method
Restore with 1.0 ml of deionized water (or equivalent).
Concentration
2.0 mg/ml (by UV absorbance at 280 nm)
Purification
Immunoaffinity Chromatography.
Conjugation
Biotin
Storage
Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
