Novel Coronavirus Spike Glycoprotein RBD (SARS-CoV-2 S RBD) IgM Antibody BioAssay™ ELISA Kit (Human) (Research Use Only)
Cat# 544481-96T
Size : 96Tests
Brand : US Biological
544481 Novel Coronavirus Spike Glycoprotein RBD (SARS-CoV-2 S RBD) IgM Antibody BioAssay™ ELISA Kit (Human) (Research Use Only)
Clone Type
PolyclonalSwiss Prot
P0DTC2Shipping Temp
RTStorage Temp
4°CThe Novel Coronavirus Spike Glycoprotein RBD (SARS-CoV-2 S RBD) IgM Antibody BioAssay™ ELISA Kit (Human) is a qualitative assay for the determination of human novel coronavirus spike glycoprotein RBD (SARS-CoV-2 S RBD) IgM antibody concentrations in serum and plasma.||Specificity:|This assay has high sensitivity and excellent specificity for detection of human novel coronavirus spike glycoprotein RBD (SARS-CoV-2 S RBD) IgM antibody. No significant cross-reactivity or interference between human novel coronavirus spike glycoprotein RBD (SARS-CoV-2 S RBD) IgM antibody and analogues was observed.|Note: Limited by current skills and knowledge, it is impossible to complete the cross-reactivity detection between human novel coronavirus spike glycoprotein RBD (SARS-CoV-2 S RBD) IgM antibody and all the analogues, therefore, cross reaction may still exist.||Test Principle (Research Use Only):|This assay employs the qualitative enzyme immunoassay technique.|The microtiter plate provided in this kit has been pre-coated with human novel coronavirus spike glycoprotein RBD. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human novel coronavirus spike glycoprotein RBD (SARS-CoV-2 S RBD) IgM antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.||Precision:|Intra-assay CV: <15%|Inter-assay CV: <15%||Kit Components:|Coated assay plate, 96Tests|Negative Control, 1x800ul|Positive Control, 1x800ul|HRP-conjugate(100X), 1x120ul|HRP-conjugate Diluent, 1x20ml|Sample Diluent, 2x20ml|Wash Buffer (25X), 1x20ml|TMB Substrate, 1x10ml|Stop Solution, 1x10ml||Storage and Stability:|Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.