Novel Coronavirus Spike Glycoprotein RBD (SARS-CoV-2 S RBD) IgM Antibody BioAssay™ ELISA Kit (Human) (Research Use Only)

Cat# 544481-96T

Size : 96Tests

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


544481 Novel Coronavirus Spike Glycoprotein RBD (SARS-CoV-2 S RBD) IgM Antibody BioAssay™ ELISA Kit (Human) (Research Use Only)

Clone Type
Polyclonal
Swiss Prot
P0DTC2
Shipping Temp
RT
Storage Temp
4°C

The Novel Coronavirus Spike Glycoprotein RBD (SARS-CoV-2 S RBD) IgM Antibody BioAssay™ ELISA Kit (Human) is a qualitative assay for the determination of human novel coronavirus spike glycoprotein RBD (SARS-CoV-2 S RBD) IgM antibody concentrations in serum and plasma.||Specificity:|This assay has high sensitivity and excellent specificity for detection of human novel coronavirus spike glycoprotein RBD (SARS-CoV-2 S RBD) IgM antibody. No significant cross-reactivity or interference between human novel coronavirus spike glycoprotein RBD (SARS-CoV-2 S RBD) IgM antibody and analogues was observed.|Note: Limited by current skills and knowledge, it is impossible to complete the cross-reactivity detection between human novel coronavirus spike glycoprotein RBD (SARS-CoV-2 S RBD) IgM antibody and all the analogues, therefore, cross reaction may still exist.||Test Principle (Research Use Only):|This assay employs the qualitative enzyme immunoassay technique.|The microtiter plate provided in this kit has been pre-coated with human novel coronavirus spike glycoprotein RBD. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human novel coronavirus spike glycoprotein RBD (SARS-CoV-2 S RBD) IgM antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.||Precision:|Intra-assay CV: <15%|Inter-assay CV: <15%||Kit Components:|Coated assay plate, 96Tests|Negative Control, 1x800ul|Positive Control, 1x800ul|HRP-conjugate(100X), 1x120ul|HRP-conjugate Diluent, 1x20ml|Sample Diluent, 2x20ml|Wash Buffer (25X), 1x20ml|TMB Substrate, 1x10ml|Stop Solution, 1x10ml||Storage and Stability:|Store kit at 4°C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.