Immunomicroscopy. Flow Cytometry or FACS analysis.
Reactivity
Mouse
Antibody Host
Chicken
Clonality
Polyclonal
Immunogen
Mouse IgG whole molecule.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, Mouse IgG and Mouse Serum.
Buffer
0,02 M Potassium Phosphate, 0,15 M Sodium Chloride, pH 7,2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0,01% (w/v) Sodium Azide as preservative. Label: TRITC State: Lyophilized purified Ig fraction. Label: Tetramethylrhodamine isothiocyanate (MW 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 3.0 moles TRITC per mol of Chicken IgG
Reconstitution Method
Restore with 1.0 ml of deionized water (or equivalent)
Concentration
lot specific
Purification
Prepared from monospecific antiserum by Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation
TRITC
Storage
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
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