Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivity
Mouse
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Mouse IgG F(ab')2 fragment.
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum. No reaction was observed against Mouse IgG F(c).
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: TRITC State: Lyophilized purified Ig fraction. Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 3.0 moles TRITC per mole of Rabbit IgG.
Reconstitution Method
Restore with 1.0 ml of deionized water (or equivalent).
Concentration
2.0 mg/ml (by UV absorbance at 280 nm)
Purification
Immunoaffinity chromatography.
Conjugation
TRITC
Storage
Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
