SNPXplex: Identity Verification Kit for High-Throughput Sequencing

Recommendations for good practice in high-throughput sequencing agree on the need for an identity verification technique, based on the comparison of the genotypes of several polymorphisms, with the genotypes being obtained independently by high-throughput sequencing (next generation sequencing, NGS) and by a second technique. The SNPXplex is an identity verification kit enabling the simultaneous genotyping of 15 single nucleotide polymorphisms (SNPs) by allele-specific fluorescent multiplex PCR and sex determination.

In use since 2017, more than 100,000 NGS sequencing results have already been secured. A technology patented by Assistance Publique - Hôpitaux de Paris (APHP) based on the comparison of genotyping results (NGS vs SNPXplex®) for several polymorphisms.
 

Key features :

Simple, rapid protocol

• Single, ready-to-use reagent
• Simultaneous genotyping of 15 single nucleotide polymorphisms (SNPs) by allele-specific fluorescent multiplex PCR + determination of sex ⇒profile
• Risk of having two patients with the same profile in a series of 96 samples:
  • <5% for all origins combined 
  • <0.2% if of European origin

Can be used with :

• Targeted panels, exomes and genomes
• Capture and amplicon

Instruments required :

• Thermal cycler
• Capillary sequencer (6-FAM™ detection)

Content 

• Reagent : SNPXplex 
• Volume: 1200 µl

Storage: 

• Reagents can be stored at -20°C. Reagents can be stored at room temperature for up to 8 days, protected from light.

Comparison of SNPXplex vs NGS genotyping results and genoidentity testing

SNPXplex genotyping data can be exported via the GeneMapper™ Export function.

Use local resources to:

• Compare SNPXplex genotyping results to high-throughput sequencing results on a patient-by-patient basis.
• Search all patients in the series for possible genoidentity between two patients.

To learn more :