IgM, Human Fc5u (Biotin)

Print

Cat# I1903-38K-500ug

Size : 500ug

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

Contact local distributor :

Phone : +1 850 650 7790

I1903-38K IgM, Human Fc5u (Biotin)

Clone Type

Polyclonal

Host

goat

Source

human

Isotype

F(ab’)2

Grade

Affinity Purified

Applications

E IC IHC WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications: |Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested.||Recommended Dilution: |ELISA: 1:50,000-1:1,000,000 |Western Blot: 1:10,000-1:100,000 |Immunohistochemistry (tissue sections)1:500-1:5000|Optimal dilutions to be determined by the researcher.||Label: |Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)||Biotin/Protein Ratio: |10-20 BAC molecules per Goat IgG molecule||Storage and Stability:|Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications

Product Type: Pab|Isotype: F(ab’)2|Host: goat|Source: human|Concentration: ~1mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder from PBS, pH 7.2, 15 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.05% Sodium Azide, 0.01% Thimerosal. Labeled with Biotin.|Purity: Purified by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |Immunogen: Human IgM Fc5µ fragment|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgM Fc5µ and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or other human heavy or light chain proteins.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human IgM Fc5µ fragment

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 15 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, 0.05% Sodium Azide, 0.01% Thimerosal. Labeled with Biotin.

Purity

Purified by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgM Fc5µ and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or other human heavy or light chain proteins.