Hoechst 33258[23491-45-4]
Cat# 40045
Size : 100MG
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
| CAS number | 23491-45-4, 23491-52-3 |
|---|---|
| Detection method/readout | Fluorescence microscopy, Flow cytometry |
| Probe cellular localization | Nucleus |
| For live or fixed cells | For fixed cells, For live/intact cells |
| Assay type/options | DNA content/cell cycle profiling by flow cytometry, Long term staining (24-72h), No-wash staining, Real-time imaging, Tissue staining |
| Cell permeability | Membrane permeant |
| Apoptosis/viability marker | All cell stain |
| Fixation options | Fix before staining (formaldehyde), Fix after staining (formaldehyde), Fix before staining (methanol), Fix after staining (methanol), Permeabilize after staining |
| Colors | Blue |
| Excitation/Emission | 352/458 nm (with DNA) |
Product Description
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Biotium offers Hoechst 33258 and Hoechst 33342 dyes in both solution or powder forms. Both dyes are spectrally similar but Hoechst 33258 is slightly more water soluble than Hoechst 33342. The dyes can be used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.
Hoechst can also be used to stain live bacteria (gram-positive and gram-negative), but in live yeast the staining is weak and not nuclear. Check out our tech tip on Hoechst & DAPI Staining, which includes helpful tips and detailed protocols for staining live and fixed cells and tissues. Also see our Cellular Stains Table for more information on how our dyes stain various organisms.
Hoechst 33258, pentahydrate
Hoechst 33342, trihydrochloride trihydrate
Biotium also offers unique NucSpot® Nuclear Stains for bright and specific nuclear staining in dead or fixed cells. The stains are available in a wide range of colors from green to near-IR. We also offer DAPI nuclear stains in powder or solution.
Hoechst Products
| Product | Catalog Number | MW | Unit Size | λEx/λEm | Format |
|---|---|---|---|---|---|
| Hoechst 33258, 10 mg/mL in Water | 40044 | 533.88 | 10 mL | 352/458 nm | Yellow solution |
| Hoechst 33258, pentahydrate | 40045 | 623.96 | 100 mg | 352/458 nm | Yellow solid |
| Hoechst 33342, 10 mg/mL in Water | 40046 | 561.93 | 10 mL | 350/461 nm | Yellow solution |
| Hoechst 33342, trihydrochloride trihydrate | 40047 | 615.98 | 100 mg | 350/461 nm | Yellow solid |
Having trouble with your experiment? See our section on troubleshooting tips for fluorescent staining.
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