H3C14 Rabbit Polyclonal Antibody
Cat# TA397482
Size : 50ug
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| Product Data | |
| Application | ChIP, IF, IHC, WB |
|---|---|
| Application | WB: 1:500 IHC: 1:50 IF: 1:50 CHiP: 2-5µg/million cells |
| Reactivity | C. elegans, Human |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Histone H3 [Dimethyl Lys9] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 9 of human Histone H3.2. |
| Specificity | Anti-Histone H3 [Dimethyl Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Gene Name | histone cluster 2, H3c |
| Database Link | |
| Background | Dimethylated histone H3 at lysine 9 is associated with several inflammatory diseases. Usually, the histone modification is implicated through a one-off association with critical disease related genes. For example, in Alzheimer's disease, amyloid beta-peptide accumulation is caused by insufficient neprilysin, which is in turn caused by increased concentrations of H3K9me2 in the neprilysin promoter following exposure to hypoxia. In osteoarthritis, increased H3K9me2 at the NFAT1 promoter represses the expression of NFAT, resulting in overexpression of proinflammatory cytokines. PHF2 and ARID5B are important in regulating the methylation of H3 at K9, and thus chromatin reorganization and gene transcription. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research. |
| Synonyms | rabbit anti-Histone H3 dimethyl Lys9 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9me2 |
| Note | Anti-Histone H3 [Dimethyl Lys9] antibody is tested for Western Blot, Chromatin Immunoprecipitation, Dot Blot, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals. |
| Reference Data | |
| Protein Pathways | Systemic lupus erythematosus |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.