This is a highly cross-adsorbed bovine anti-goat IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins.

• Highly cross-adsorbed for specific staining with minimal background
• Minimal cross-reactivity with milk or BSA blocking solutions
• Available in 7 bright and photostable CF® Dyes
• Suitable for western, immunofluorescence, and immunohistology

Bovine host minimizes anti-goat (IgG) cross-reactivity with bovine IgG

Bovine serum albumin (BSA) and dry milk are used extensively for blocking non-specific binding of antibodies and for stabilizing antibodies and other proteins during freeze drying and in dilute solutions. However dry milk and most commercial sources of BSA contain bovine IgG which interferes with the use of anti-goat (IgG) secondary antibodies due to the close phylogenetic relationship between cows and goats. This causes non-specific background which may lead to false results and needless repetition of experiments. This antibody is created by immunizing a cow host with goat IgG which helps minimize cross-reactivity with bovine IgG and therefore reduce background from BSA containing solutions.

Bovine Anti-Goat IgG (H+L), Highly Cross-Adsorbed

View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH₂O

HRP or DNP conjugates: add 1 mL dH₂O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

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