CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (HRP)

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Cat# C2399-07K-HRP-100ul

Size : 100ul

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

C2399-07K-HRP CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (HRP)

Clone Type

Polyclonal

Host

mouse

Source

guinea pig

Isotype

IgG1

Grade

Affinity Purified

Applications

IHC IP

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:|Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications not tested.||Recommended Dilution:|Immunohistochemistry: frozen sections|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: IH-1|Host: mouse|Source: guinea pig|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Guinea pig leukemic B lymphocyte L2C cells.|Specificity: Recognizes CD45 on all guinea pig leukocytes. Immunoprecipitates proteins of MW: 220,000, 210,000 and 195,000 and 180,000 from lymph node lymphocytes and proteins of MW 195,000, 180,000 and 170,000 from thymocytes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Guinea pig leukemic B lymphocyte L2C cells.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes CD45 on all guinea pig leukocytes. Immunoprecipitates proteins of MW: 220,000, 210,000 and 195,000 and 180,000 from lymph node lymphocytes and proteins of MW 195,000, 180,000 and 170,000 from thymocytes.

References

1.Hart, I.J. et al. (1992) Subpopulations of guinea-pig T lymphocytes defined by isoforms of the leucocyte common antigen. Immunology. 77 (3): 377-84.2.Wang, Y. et al. (2010) Local host response to chlamydial urethral infection in male guinea pigs. Infect Immun. 78: 1670-81. 3.Shang, S. et al. (2011) Activities of TMC207, rifampin, and pyrazinamide against Mycobacterium tuberculosis infection in guinea pigs. Antimicrob Agents Chemother. 55 (1): 124-31.4.Lacy, H.M. et al. (2011) Essential role for neutrophils in pathogenesis and adaptive immunity in Chlamydia caviae ocular infections. Infect Immun. 79 (5): 1889-97.Tura-Ceide, O. et al. (2017) Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig. Respir Res. 18 (1): 50. Ishikawa, M. et al. (2017) Transplantation of neurons derived from human iPS cells cultured on collagen matrix into guinea-pig cochleae. J Tissue Eng Regen Med. 11 (6): 1766-78.