ATP4B (NM_000705) Human Tagged ORF Clone
Cat# RC206563
Size : 10µg
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ATP4B (NM_000705) Human Tagged ORF Clone
SKU
RC206563
ATP4B (Myc-DDK-tagged)-Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B)
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ATP4B |
| Synonyms | ATP6B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC206563 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTCTGCAGGAGAAGAAGACGTGTGGCCAGCGCATGGAGGAGTTCCAGCGTTACTGCTGGAACC CGGACACGGGGCAGATGCTGGGCCGCACCCTGTCCCGGTGGGTGTGGATCAGCCTGTACTACGTGGCCTT CTACGTGGTGATGACTGGGCTCTTCGCCCTGTGCCTCTATGTGCTGATGCAGACAGTGGACCCGTACACA CCGGACTACCAAGACCAGCTACGGTCACCAGGGGTAACCTTAAGGCCGGATGTTTACGGGGAGAAAGGCC TGGAAATTGTCTACAACGTCTCTGATAACAGAACCTGGGCAGACCTCACACAGACTCTCCACGCCTTCCT AGCAGGCTACTCTCCAGCAGCCCAGGAGGACAGCATCAACTGCACCTCCGAGCAGTACTTCTTCCAGGAG AGTTTCCGCGCTCCCAACCACACCAAGTTCTCCTGCAAGTTCACGGCAGATATGCTGCAGAACTGCTCAG GCCTGGCGGATCCCAACTTCGGCTTTGAAGAAGGAAAGCCATGTTTTATTATTAAAATGAACAGGATCGT CAAGTTCCTCCCCAGCAACGGCTCGGCCCCCAGAGTGGACTGCGCCTTCCTGGACCAGCCCCGCGAGCTC GGCCAGCCGCTGCAGGTCAAGTACTACCCTCCCAACGGCACCTTCAGTCTGCACTACTTCCCTTATTACG GGAAGAAAGCCCAGCCCCACTACAGCAACCCCCTGGTGGCAGCGAAGCTCCTCAACATCCCCAGGAACGC TGAGGTCGCCATCGTGTGCAAGGTCATGGCAGAGCACGTGACCTTCAACAATCCCCACGACCCGTATGAA GGGAAAGTGGAGTTCAAACTCAAGATTGAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC206563 protein sequence Red=Cloning site Green=Tags(s) MAALQEKKTCGQRMEEFQRYCWNPDTGQMLGRTLSRWVWISLYYVAFYVVMTGLFALCLYVLMQTVDPYT PDYQDQLRSPGVTLRPDVYGEKGLEIVYNVSDNRTWADLTQTLHAFLAGYSPAAQEDSINCTSEQYFFQE SFRAPNHTKFSCKFTADMLQNCSGLADPNFGFEEGKPCFIIKMNRIVKFLPSNGSAPRVDCAFLDQPREL GQPLQVKYYPPNGTFSLHYFPYYGKKAQPHYSNPLVAAKLLNIPRNAEVAIVCKVMAEHVTFNNPHDPYE GKVEFKLKIEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms  Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map  |
| ACCN | NM_000705 |
| ORF Size | 873 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000705.1 |
| RefSeq Size | 1509 bp |
| RefSeq ORF | 876 bp |
| Locus ID | 496 |
| UniProt ID | P51164 |
| Cytogenetics | 13q34 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Oxidative phosphorylation |
| MW | 33.4 kDa |
| Summary | The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008] |
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC206563L1 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B), Myc-DDK-tagged | 10 ug | | |
| RC206563L2 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B), mGFP tagged | 10 ug | | |
| RC206563L3 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B), Myc-DDK-tagged | 10 ug | | |
| RC206563L4 | Lenti ORF clone of Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B), mGFP tagged | 10 ug | | |
| RG206563 | ATP4B (tGFP-tagged) - Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B) | 10 ug | MSRP MSRP | |
| SC122571 | ATP4B (untagged)-Human ATPase, H+/K+ exchanging, beta polypeptide (ATP4B) | 10 ug | | |
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