Anti-Pectic polysaccharide, beta-1,4-galactan (monoclonal, clone LM5)
Cat# AS184197-1ml
Size : 1ml
Brand : Agrisera
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Phone : +1 850 650 7790
Anti-Pectic polysaccharide, beta-1,4-galactan (monoclonal, clone LM5)
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
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| Sub class: | IgG |
| Immunogen: | Pectic polysaccharide (beta 1,4-galactan) |
| Host: | Rat |
| Clonality: | Monoclonal |
| Purity: | Cell culture supernatant. |
| Format: | Liquid |
| Quantity: | 1 ml |
| Storage: | Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube. |
| Tested applications: | ELISA (ELISA), Immunoflourescence (IF) |
| Recommended dilution: | 1:10 (ELISA, IF) |
| Confirmed reactivity: | Higher plants, ferns and mosses |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
 Samples:
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| Additional information: | Contains 0.05% Sodium Azide. No cross-reactivity with (1-3)-beta-D-galactans or (1-6)-beta-D-galactans. It recognizes a linear tetrasaccharide in (1-4)-beta-D-galactans. In ELISA (competitive inhibition), antibody is binding to: (1-4)-beta-D-galactan was inhibited (50%) by 58 µg/ml (1-4)-beta-D-galactotetraose and by 0.7 µg/ml lupin (1-4)-beta-D-galactan. |
| Background: | The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls. |
| Selected references: | Andersen et al. (2016). Characterization of the LM5 pectic galactan epitope with synthetic analogues of ß-1,4-d-galactotetraose. Carbohydr Res. 2016 Dec ;436:36-40.doi: 10.1016/j.carres.2016.10.012. Jones et al. (1997). Development and validation of an in vitro model system to study peripheral sensory neuron development and injury. Sci Rep. 2018 Oct 29;8(1):15961. doi: 10.1038/s41598-018-34280-3. |