Anti-Pectic polysaccharide, alpha-1,5-arabinan (monoclonal, clone LM6)

Cat# AS184198-1ml

Size : 1ml

Brand : Agrisera

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Anti-Pectic polysaccharide, alpha-1,5-arabinan (monoclonal, clone LM6)

Product no: AS18 4198-1ml
AS18 4198-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
    Product Info
    Immunogen: Pectic polysaccharide (alpha-1,5-arabinan), neoglycoprotein (arabinoheptaose-BSA conjugated),
    Sub class: IgG
    Host: Rat
    Clonality: Monoclonal
    Purity: Cell culture supernatant.
    Format: Liquid
    Quantity: 1 ml
    Storage: Store at +4°C (short term) and at -20°C (long term). Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunoflourescence (IF)
    Recommended dilution: 1:10 (ELISA, IF)
    Reactivity
    Confirmed reactivity: Higher plants, ferns and mosses
    Additional Information
    Additional information: Contains 0.05% Sodium Azide

    No cross-reactivity with gum arabic. The antibody recognises a linear pentasaccharide in (1-5)-α-L-arabinans. I many species it can also recognise pectic polysaccharides.
    In some species this antibody could recognize arabinogalactan-proteins (AGPs).

    In competitive inhibition ELISAs, antibody is binding to: (1-5)-α-L-arabinan was inhibited (50%) by 40 ng/ml (1-5)-α-L-arabinopentaose and 19 ng/ml (1-5)-α-L-arabinohexaose.
    Background
    Background:

    The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Pectins are the major polysaccharides that build pectic matrix of plant primary cell walls.


    Product Citations
    Selected references: Verhertbruggen et al. (2009). Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. Plant J. 2009 Aug;59(3):413-25.doi: 0.1111/j.1365-313X.2009.03876.x.
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