Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using RM224 at 0.5 ug/mL, showed a band of Histone H2A.X phosphorylated at serine 139 in HeLa cells. Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green). Immunohistochemical staining of FFPE Lung cancer with radiotherapy tissues, Using anti-gamma-H2AX [RM224] rabbit monoclonal antibodies. IHC Staining Comparison of FFPE normal lung, Lung cancer without chemotherapy & radiotherapy, Lung cancer with chemotherapy, Lung cancer with radiotherapy tissues, Using anti-DNA-PKcs (total) [RM505], anti-phospho-DNA-PKcs (Ser2056) [RM500], anti-gamma-H2AX [RM224] rabbit monoclonal antibodies.
Product Details Product Name Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Clone RM224 Product Description Rabbit monoclonal to Gamma H2AX (phospho-Ser139) Catalog No. 31-1104-00 Clone Name RM224 Product Data Sheet Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody Data Sheet RM224 Specificity RM224 reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones. Immunogen A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139). Application Western Blot (WB)
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt. Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 μg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot: 0.5 μg/mL – 2 μg/mL;
ELISA: 0.2 μg/mL - 1 μg/mL;
Multiplex: 0.1 μg/mL – 1 μg/mL;
ICC: 0.5 μg/mL - 2 μg/mL.
Keywords . Product Images 
Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody [RM224]
![Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody [RM224]](https://www.biotrend.com/en/upload/-img-lapin1.png)
Cat# 31-1104-00-S
Size : 25ug
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody [RM224]
![Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody; Histone Gamma-H2A.X (Phospho S139) [Clone RM224], 31-1104-00](https://www.revmab.com/wp-content/uploads/2024/08/Fig-RM224-Gamma-H2AX-Lung-Cancer-with-Radiotherapy-510x383.jpg)
![Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody; Histone Gamma-H2A.X (Phospho S139) [Clone RM224], 31-1104-00 - Image 2](https://www.revmab.com/wp-content/uploads/2016/01/Fig1_WB_gH2AX_e-510x510.png)
![Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody; Histone Gamma-H2A.X (Phospho S139) [Clone RM224], 31-1104-00 - Image 3](https://www.revmab.com/wp-content/uploads/2016/01/Fig2_ICC_gH2AX_e-510x510.png)
![Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody; Histone Gamma-H2A.X (Phospho S139) [Clone RM224], 31-1104-00 - Image 4](https://www.revmab.com/wp-content/uploads/2024/08/RevMAb-DNA-damage-marker-RM224-RM500-RM505-watermark-510x306.jpg)
![Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody; Histone Gamma-H2A.X (Phospho S139) [Clone RM224], 31-1104-00 - Image 2](/proxy_img/aHR0cHM6Ly93d3cucmV2bWFiLmNvbS93cC1jb250ZW50L3VwbG9hZHMvMjAxNi8wMS9GaWcxX1dCX2dIMkFYX2UucG5n.png)
![Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody; Histone Gamma-H2A.X (Phospho S139) [Clone RM224], 31-1104-00 - Image 3](/proxy_img/aHR0cHM6Ly93d3cucmV2bWFiLmNvbS93cC1jb250ZW50L3VwbG9hZHMvMjAxNi8wMS9GaWcyX0lDQ19nSDJBWF9lLnBuZw==.png)
Rabbit monoclonal to Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Histone Gamma-H2A.X (Phospho S139)
25 µg Trial Size, 100 µg Full Size
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