75-147 | Anti-Ankyrin-G (Staining) Antibody Biotrend

Anti-Ankyrin-G (Staining) Antibody (N106/65)

Our Anti-Ankyrin-G (Staining) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N106/65. It detects human, mouse, and rat Ankyrin-G (Staining), and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Representative images showed the high expression level of FKBP12 (green) in axonal hillock/axon initial segment (labeled by anti–ankyrin-G antibody, cat. 75-147; bright blue) under basal conditions whereas FKBP12 translocated to soma and dendrites when neurons bear tau aggregation. Image from publication CC-BY-4.0. PMID:36724228

Immunofluorescence labeling of axon initial segments in adult rat cortex with N106/65 TC supe (red) and Hoechst nuclear stain (blue).

Species Reactivity

Human, Mouse, Rat

Applications

EM, ICC, IHC

Host Species

Mouse

Data Sheet

Trial Size

SKU: 75-147

Product Details

Target

Ankyrin-G (Staining)

Target Description

Ankyrin-G (ankyrin 3) is a member of the Ankyrin family which consists of Ankyrin R (1), Ankyrin B (2) and Ankyrin G (3) all of which are expressed in the nervous system. Ankyrins are structural proteins that aid in the attachment of integral membrane proteins with the spectrin-actin cytoskeleton, and thus targets voltage-gated sodium channels, potassium channels, cell adhesion molecules and others at the nodes of Ranvier and axonal initial segments (AIS). Variants in Ankyrin G have been linked to bipolar disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N106/65

Isotype

IgG2b

Applications

EM, ICC, IHC

Host Species

Mouse

Gene Name

ANK3

Molecular Weight

<200 kDa

Antigen

Fusion protein ~1000 amino acids of Ankyrin-G (accession number E9PE32) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10675130

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.74

Dilution Ranges

IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with Ankyrin-B

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Ankyrin-3 (ANK-3) (Ankyrin-G)

UniProt Details

UniProt (Human): Q12955
UniProt (Immunogen Species): E9PE32

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Human | Mouse
Western Blot: Human | Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
32732889	1:100	Yang, C., et al. 2020. Targeting QKI-7 in vivo restores endothelial cell function in diabetes. Nature Communications, 3812.

Immunohistochemistry: Human
PMID	Dilution	Publication
27029405	10ug/ml	de Bruin, R.G., et al. 2014. Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression.. Nature Communications, 10846.
23963726	not listed	van der Veer, E.P., et al. 2013. Quaking, an RNA-binding protein, is a critical regulator of vascular smooth muscle cell phenotype.. Circulation Research, 1065-1075.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
38985877	not listed	Früh, S., et al. 2024. Monoallelic de novo AJAP1 loss-of-function variants disrupt trans-synaptic control of neurotransmitter release. Science Advances, eadk5462.
36830639	1:500	De Nuccio, F., et al. 2023. Oligodendrocytes Prune Axons Containing α-Synuclein Aggregates In Vivo: Lewy Neurites as Precursors of Glial Cytoplasmic Inclusions in Multiple System Atrophy?. Biomolecules, 269.
27454326	1:100	Bin, J.M., et al. 2016. The oligodendrocyte-specific antibody ''CC1'' binds Quaking 7.. Journal of Neurochemistry, 181-186.
27196066	not listed	Suiko, T., et al. 2016. Expression of Quaking RNA-Binding Protein in the Adult and Developing Mouse Retina. PLoS One, e0156033.

Western Blot: Human
PMID	Dilution	Publication
35544276	not listed	Fagg, W.S., et al. 2022. Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate. Nucleic acids research, 5313-5334.
24449917	1:1000	Chan, C.B., et al. 2014. PIKE is essential for oligodendroglia development and CNS myelination.. PNAS: USA, 1993-1998.

Western Blot: Mouse
PMID	Dilution	Publication
32732889	1:1000	Yang, C., et al. 2020. Targeting QKI-7 in vivo restores endothelial cell function in diabetes. Nature Communications, 3812.
27454326	1:1000	Bin, J.M., et al. 2016. The oligodendrocyte-specific antibody ''CC1'' binds Quaking 7.. Journal of Neurochemistry, 181-186.
27196066	not listed	Suiko, T., et al. 2016. Expression of Quaking RNA-Binding Protein in the Adult and Developing Mouse Retina. PLoS One, e0156033.

Anti-Ankyrin-G (Staining) Antibody

Cat# 75-147

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Brand : Antibodies Incorporated

Contact local distributor :

Anti-Ankyrin-G (Staining) Antibody (N106/65)

SKU: 75-147

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Ankyrin-G (Staining) Antibody

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