Angiotensin Converting Enzyme (ACE1) Activity Assay Kit
Cat# E-BC-K003-M-96T
Size : 96T
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| Detection Principle | N-(3-(2-furyl) acryloyl)-L-phenylalanyl-glycyl-glycine (FAPGG) have the maximum absorption peak at 340 nm. ACE1 catalyzes hydrolysis of FAPGG substrate to furanyl phenylalanine (FAP) and glycylglycine (GG), and the absorbance at 340 nm will be decreased. The activity of ACE1 can be calculated indirectly by measuring the decrease in absorbance at 340 nm. |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Metabolic Diseases |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 25 U/L |
| Detection Range | 25-620 U/L |
| Precision | inter-assay CV: 2.9-5.2% | intra-assay CV: 1.8-3.1% |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| Rat plasma | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 3-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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