Alcohol Dehydrogenase (ADH) Activity Assay Kit

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Cat# E-BC-K763-M-96T

Size : 96T

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Detection Principle	ADH catalyzes the oxidative dehydrogenation of ethanol. Meanwhile, NAD⁺ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of ADH can be calculated by measuring the change of absorbance value at 450 nm.
Synonyms	ADH
Sample Type	Serum,Plasma,Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Research Area	Liver And Renal Function
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.29 U/L
Detection Range	0.29-248 U/L
Precision	inter-assay CV: 4.5% \| intra-assay CV: 4.2%
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-K235-M	Alanine Aminotransferase (ALT/GPT) Activity Assay Kit	96T , 48T
E-BC-K236-M	Aspartate Aminotransferase (AST/GOT) Activity Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat lung tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Rat liver tissue homogenate	2-4
10% Rat heart tissue homogenate	1-3
10% Mouse liver tissue homogenate	2-4
10% Rat brain tissue homogenate	1-3
Mouse serum	1
Porcine serum	1
Human serum	1
Dog serum	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.