AccuOrange™ Protein Quantitation Kit, 2000 assays
Cat# 30071
Size : 1kit
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
AccuOrange™ Protein Quantitation Kit
Highly sensitive, fluorescence-based quantitation of purified proteins.
Product Attributes
| Excitation/Emission | 480/598 nm |
|---|---|
| Storage Conditions | Store at room temperature, Do not refrigerate, Protect dye component from light |
Product Description
AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantifying purified protein samples in 96-well format.
- Highly sensitive: detect 0.1-15 ug/mL protein
- Excellent linearity and low variability
- Stable fluorescence signal
- Compatible with reducing agents and other additives
- For use with purified protein or antibody samples
- Ex/Em: 480/598 nm
The detection range of the assay is 0.1-15 ug/mL protein. AccuOrange™ is much more sensitive than traditional protein quantitation assays such as BCA, Bradford and Lowry, and shows superior linearity and reproducibility compared to the NanoOrange® protein quantitation assay. The assay shows minimal variability between different proteins, and has stable fluorescence signal for up to 16 hours.
AccuOrange™ is recommended for quantifying purified protein or antibody samples. The tolerance of the AccuOrange™ assay to salts, buffers, detergents, and other chemicals is listed in the product protocol. The AccuOrange™ assay has low tolerance for non-ionic detergents, and is not recommended for use with cell lysates containing Triton™ X-100, sodium deoxycholate, CHAPs, or other non-ionic detergents. The assay can tolerate up to 0.01% SDS (final concentration in assay).
Comparison of AccuOrange™ with other protein quantitation assays
| Assay | Detection Range | Comments |
|---|---|---|
| AccuOrange™ | 0.1-15 ug/mL | Fluorescence-based (480/598 nm) Highly linear Signal stable for at least 16 hours Compatible with reducing agents Not compatible with detergents |
| NanoOrange® | 0.1-10 ug/mL | Fluorescence-based (470/570 nm) Non-linear Fluorescence stable for 6 hours Compatible with reducing agents Not compatible with detergents |
| Modified Lowry | 1-1500 ug/mL | Absorbance-based (750 nm) Non-linear Not compatible with reducing agents or detergents |
| BCA | 20-2000 ug/mL | Absorbance-based (562 nm) Highly linear Signal not stable over time Not compatible with reducing agents Compatible with detergents |
| Bradford (Coomassie®) | 50-500 ug/mL | Absorbance-based (595 nm) Signal not stable over time Non-linear Compatible with reducing agents Not compatible with detergents |
| Pierce® 660 nm | 50-2000 ug/mL | Absorbance-based (660 nm) Non-linear Compatible with reducing agents & detergents |
| A280 | 50-2000 ug/mL | Absorbance-based (280 nm) High protein-protein variability Contaminants and detergents can affect results |
NanoOrange is a registered trademark of Thermo Fisher Scientific. Triton is a trademark of The Dow Chemical Company.
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Cat#
Description
Cond.
Price Bef. VAT
31028
1kit