HRP Chromogen for IHC - AEC
AEC (Aminoethyl carbazole) is one of the most used chromogens for immunohistochemistry (IHC) using horseradish peroxidase (HRP). HRP catalyzes the formation of a red precipitate from AEC.
The AEC is easy to use, simply incubate the slides for 5-10 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under microscope.
Since AEC is soluble in alcohol, samples should not be dehydrated after staining.
Slides can be mounted using an aqueous mounting medium (Simpo-Mount, NeoMount ...).
AEC can be used as a contrast stain with tissue pigments or for multiple labeling with other chromogens.
Name
AEC (Aminoethyl carbazole)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Red
Microscopy
Brightfield, Spectral Imaging
Mounting
Aqueous
Other properties
Contrast with Tissue Pigments, Multiple Labeling
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RUOCE / IVD
- Unconjugated 2055
- HRP 7
- APC 6
- Biotin 6
- FITC 6
- MaxLight 490 6
- MaxLight 550 6
- MaxLight 650 6
- PE 6
- MaxLight 405 4
- MaxLight 750 4
- human 79
- cow 67
- bacteria 63
- pig 40
- primary 15
- mouse 14
- e. coli 10
- rat 10
- yeast 6
- chicken 4
- dog 4
- guinea pig 4
- monosan aec substrate 2
- 1 1
- monosan permanent aec 1
- monosan plus (hrp) broad spectrum (aec)kit 1
- yeast 1435
- 850
- e. coli 238
- mouse 62
- rabbit 39
- bacteria 38
- rat 9
- human 8
- dog 2
- goat 1
- virus 1
- Protein/peptide 5933
- Biochemicals 105
- Primary antibody 73
- ELISA/ELISPOT 40
- Inhibitor/Antagonist/Agonist 33
- Cell line 20
- Primary cells 15
- Buffers and reagents 13
- kit 7
- pcr products 6
- ELISA 66
- WB 64
- IHC 52
- IF 39
- FLISA 16
- PCR 6
- ICC 4
- FC 3
- IP 1
- 5C4 32
- 2F7 30
- AAEC-19 1
- AEC-16 1
- AECB-3 1
- AECC-14 1
- AECE-1 1
- AECF-19 1
- AECG-9 1
- AECH-22 1
- AECI-19 1
- AECO-3 1
- Polyclonal 1
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