Viability PCR starter kits contain the materials that you need for selective detection of viable cells using either PMA or PMAxx™ viability dye and real-time PCR. The kits contain Forget-Me-Not™ EvaGreen® qPCR Master Mix, and are available with or without Enhancer for gram-negative bacteria. The user will need to supply primers to amplify DNA from their species of interest (see this

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About this kit

This kit contains reagents sufficient to treat 80 cultures with PMA or PMAxx™, and perform 200 PCR reactions. There is an option to choose either PMA or PMAxx™ for the viability dye. Original PMA dye has been validated for vPCR in hundreds of publications and a wide variety of bacteria, yeast, and viruses. PMAxx™ is a next-generation vPCR dye designed by Biotium scientists to provide superior live/dead discrimination compared to PMA.

Kit options are available with of PMA Enhnacer for Gram-Negative Bacteria, a reagent that improves live/dead discrimination for gram-negative bacteria (note: Enhancer is not recommended for use with gram-positive bacteria or samples with mixed strains).

To learn more about the advantages of determining microbial or cell viability using viability PCR, visit the

Viability PCR Technology Page

PMA/PMAxx™ Reference List

Validated Bacterial Strains List