TransNGS® Fast RNA-Seq Library Prep Kit for Illumina®

Katalog-Nummer KP701-01

Size : 12rxns

Marke : TransGen Biotech

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Telefonnummer : +1 850 650 7790

TransNGS® Fast RNA-Seq Library Prep Kit for Illumina®

Catalog Number: KP701-01

Price:Please inquire first

Specification:
12 rxns
96 rxns

Product Details

The kit is designed for the Illumina high-throughput sequencing platform to preparestrand-specific or  non-strand-specific transcriptome libraries. The applicable sample is 0.1 ng-100 ng of processed RNA (mRNA  captured by mRNA magnetic beads or RNA obtained after rRNA removal) to prepare a high-quality sequencing  library with high yield and complete information. The kit completes the second-strand cDNA synthesis, end  repair and A-tailing in one step, without the purification steps, which greatly simplifies the operation process  and shortens the library preparation time, and can complete the preparation of high-quality sequencing libraries  within 3 hours .

Features

• No actinomycin D, no need to avoid light, non-toxic operation, higher strand-specific ratio. 

• High library conversion rate, high library yield, and high data quality. 

Applications

 • Whole transcriptome sequencing 

 • Gene expression analysis 

 • Single nucleotide variation analysis 

 • Variable splicing testing 

 • Fusion genetic testing 

 • Analysis of non-coding RNA and RNA precursor

Storage

at -20°C for one year

Shipping

Dry ice (-70℃)

Product Composition

Component

   KP701-01(12 rxns) KP701-02(96 rxns)
1×RNA Fragmentation Buffer 
230 μl 2×890 μl 
Library First-Strand Buffer III 75 μl 580 μl  
Library First-Strand Enzyme Mix II 24 μl 
192 μl 
Library SEA Buffer III (dUTP) 300 μl 4×600 μl 
Library SEA Buffer III (dNTP) 300 μl 4×600 μl
Library SEA Enzyme Mix II 120 μl 
960 μl 
TransNGS® Adapter for Illumina® (16 μM) 60 μl 480 μl   
Adapter Dilution Buffer 600 μl 5 ml 
Adapter Ligation Buffer IV 360 μl  4×720 μl  
Adapter Ligation Enzyme III 60 μl  480 μl
TransNGS® Library Amplification SuperMix (2×)  300 μl 4×600 μl
TransNGS® Universal Primer for Illumina 60 μl480 μl 
Uracil-DNA Glycosylase12 μl 96 μl
RNase-free Water2×1 ml
3×5 ml


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