TransNGS® DNA Library Prep Kit for Illumina®

Katalog-Nummer KP201-03-V2

Size : 96rxns

Marke : TransGen Biotech

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TransNGS® DNA Library Prep Kit for Illumina®

Catalog Number: KP201-11-V2

Price:Please inquire first

Specification:
12 rxns
96 rxns

Product Details

TransNGS® DNA Library Prep Kit for Illumina® is developed for the Illumina high-throughput sequencing  platform, designed to efficiently and rapidly construct sequencing libraries from 1 ng to 1 μg of fragmented  dsDNA. This reagent kit is suitable for various types of samples, including sonication fragmented genomic  DNA, enzymatic digests, nucleic acid amplification products, chromatin immunoprecipitation DNA (ChIP  DNA), and DNA from paraffin-embedded tissue sections (FFPE DNA), among others. It can be used in  conjunction with exon capture or other target capture reagents (including Agilent SureSelect® and Roche  NimbleGen SeqCap® EZ series) for exon sequencing or targeted sequencing.

Features

 • Wide range of applicable sample types.

 • High library conversion rate. 

Applications

• Whole genome sequencing. 

• Targeted gene sequencing.

• Exon sequencing / other target capture sequencing. 

• Metagenomics sequencing. 

• Immunoprecipitation sequencing.

Storage

at -20℃ for one year

Shipping

Dry ice (-70℃) 

Product Composition

Component

    KP201-11-V2 (12 rxns) KP201-03-V2 (96 rxns)
End-repair and A-tailing Reaction Mix 120 μl  960 μl 
End-repair and A-tailing Enzyme Mix 60 μl 480 μl  
TransNGS® Adapter for Illumina® (16 μM)* 60 μl 480 μl  
Adapter Dilution Buffer 600 μl  
5 ml 
Adapter-ligation Buffer III 360 μl4×720 μl 
Adapter-ligation Enzyme II 60 μl480 μl 
TransNGS® Library Amplification SuperMix (2×) 300 μl 4×600 μl
TransNGS® Universal Primer Mix for Illumina®60 μl 480 μl
Library Elution Buffer 2 ml  4×4 ml 
Nuclease-free Water1 ml
5 ml


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