Size : 500ul
Telefonnummer : +1 850 650 7790
pEASY®-T1 Cloning Kit
Catalog Number： CT101-01
Price：Please inquire first
pEASY®-T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products.
•5 minutes fast ligation of Taq-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months
Shipping
Dry ice (-70℃)
| Component | CT101-01 (20 rxns) | CT101-02 (60 rxns) |
| pEASY ®-T1 Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
1 Lu S, Luo S, Liu C, et al. Induction of significant neutralizing antibodies against SARS-CoV-2 by a highly attenuated pangolin coronavirus variant with a 104nt deletion at the 3'-UTR[J]. Emerging Microbes & Infections, 2023.(IF 19.56)
2 Li Y, Xu J, Guo X, et al. The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model[J]. Genome Biology, 2023.(IF 17.90)
3 Shi Y, Xu M, Duan X, et al. WarmStart colorimetric loop-mediated isothermal amplification for the one-tube, contamination-free and visualization detection of Shigella flexneri[J]. International Journal of Infectious Diseases, 2021.(IF 12.07)
4 Wang S, Teng D, Li X, et al. The evolution and diversification of oakleaf butterflies[J]. Cell, 2022.(IF 66.85)
5 Zhang Q, Zhang X, Zhu Y, et al. Recognition of cyclic dinucleotides and folates by human SLC19A1[J]. Nature, 2022.(IF 69.50)
6 Xia J, Guo Z, Yang Z, et al. Whitefly hijacks a plant detoxification gene that neutralizes plant toxins[J]. Cell, 2021.(IF 38.63)
7 Liu Q, Deng S, Liu B, et al. A helitron-induced RabGDIα variant causes quantitative recessive resistance to maize rough dwarf disease[J]. Nature Communications, 2020.(IF 12.12)
