Sumo 1 (SUMO1) Mouse Monoclonal Antibody [Clone ID: 5B12]
Specifications
Product Data | |
Clone Name | 5B12 |
Applications | IF, WB |
Recommended Dilution | Western blot: 1 μg/ml for chemiluminescence detection system. |
Reactivities | Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Recombinant full-length GST-SUMO-1 |
Specificity | This antibody reacts with SUMO-1 (15~17kDa). |
Formulation | PBS containing 50% glycerol, pH 7.2. Contains no preservatives. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store (in aliqouts) at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | small ubiquitin-like modifier 1 |
Database Link | |
Background | Sumoylation, the covalent attachment of a small ubiquitin-like modifier (SUMO) peptide to lysine residues of targeted substrate, has recently emerged as an important mechanism in transcriptional control. The 15~17 kDa SUMO-1/UBL1/Sentrin is processed by SUMO hydrolase / isopeptidase to generate a free glycine residue that covalently attaches to protein substrates. Unlike ubiquitin, SUMO-1 does not appear to target proteins for degradation but seems to be involved in the modulation of protein-protein interactions. SUMO modification represses the activity of targeted transcriptional activators by altering their subcompartmentalization and binding properties. Sumoylation also recruits histone deacetylases, leading to SUMO-dependent transcriptional repression. Major SUMO-1 substrates include RanGAP1, PML, SP100, p53, Mdm2, c-Jun, topoisomerase I and II, and Iκ B. |
Synonyms | SMT3C, SMT3H3, UBL1, GMP1, SMT3 homolog 3, Sentrin |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Immunofluorescence microscopy |
Reference Data |
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