SULT4A1 (Sulfotransferase family 4A, Member 1, Brain sulfotransferase-like protein, Nervous system sulfotransferase, brain sulphotransferase-like, nervous system cytosolic sulfotransferase, sulfotransferase-related protein)

SULT4A1 (Cytosolic Sulfotransferase 4A1; also BR-STL1/Brain sulfotransferase-like protein) is a 33-36kD member of the sulfotransferase 4 family of enzymes. SULT4A1 message is widely expressed. However, it appears to be translated only in brain. SULT4A1 is very unusual in that it is the only SULT that shows minimal activity on synthetic laboratory substrates. Instead, it acts on catecholamines and T4 in a manner that may not involve sulfonation. Human SULT4A1 is 284aa in length. A weak PAPS binding site is found between aa246-254. There are two potential isoforms, one that shows a 13aa substitution for aa248-284, and a second that contains a 48aa substitution for aa202-247. Full-length human SULT4A1 is 98% aa identical to mouse SULT4A1.||Applications: |Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.||Recommended Dilution:|ELISA: 0.5-1ug/ml|Western Blot: 1ug/ml|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.