Sucrose Phosphorylase (Biotin)

Applications: |Suitable for Immunoblot (Western or Dot Blot), ELISA, Immunomicroscopy and Immunohistochemistry as well as other antibody based assays using streptavidin or avidin conjugates. Other applications not tested.||Recommended Dilution:|ELISA: 1:25,000-1:110,000; 1ug of Sucrose Phosphorylase was used in a standard capture ELISA with Streptavidin (HRP) S7973-97A and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]), A0030, as a substrate for 30 minutes at RT.|Optimal dilutions to be determined by the researcher.||Label: |Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)||Biotin/Protein Ratio: |10-20 BAC molecules per Goat IgG molecule||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.