SIRT1, 254-495aa, Human, His tag, E.coli
Katalog-Nummer 01-P2625-500ug
Size : 500ug
Marke : American Research Products
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SIRT1, 254-495aa, Human
Category: Proteins
Catalog
01-P2625
| Product Name | SIRT1, 254-495aa, Human |
|---|---|
| Description | SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Alternative splicing results in multiple transcript variants. Recombinant human SIRT1 protein, fused to His-tag at N-terminus, was expressed in E.coli . |
| Synonyms | Sirtuin 1, Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae), SIR2L1 |
| Host | E. coli |
| Molecular Weight | 11.1 kDa(101aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMKK IIVLTGAGVS VSCGIPDFRS RDGIYARLAV DFPDLPDPQA MFDIEYFRKD PRPFFKFAKE IYPGQFQPSL CHKFIALSDK EGKLLRNYTQ NIDTLEQVAG IQRIIQCHGS FATASCLICK YKVDCEAVRG DIFNQVVPRC PRCPADEPLA IMKPEIVFFG ENLPEQFHRA MKYDKDEVDL LIVIGSSLKV RPVALIPSSI PHEVPQILIN REPLPHLHFD VELLGDCDVI INELCHRLGG |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90 % by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Langley E., et al. (2002) EMBO J. 21:2383-2396. |
| Background | SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. Alternative splicing results in multiple transcript variants. Recombinant human SIRT1 protein, fused to His-tag at N-terminus, was expressed in E.coli . |
| Supplier | ARP |
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