Description: Red Blood Cell Lysis Buffer is mainly used for the detection of anticoagulated whole blood samples by flow cytometry. Form: Red Blood Cell Lysis Buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. Directions for Use : 1. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use. 2. Add 2.0 ml of 1X Red Blood Cell Lysis Buffer to each tube containing up to 100 µl of whole blood. 3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes. 4. Centrifuge 1000rpm for 5 minutes. Aspirate supernatant without disturbing pellet. 5. Resuspend the pellet in the appropriate buffer, wash 1X. 6. Resuspend and proceed with further procedures. Application : The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use. Storage/Stability : Store at 4°C for one year. Do not freeze.