P1372-500ug | Recombinant Rat Interleukin-1 Receptor Antagonist Protein

Background

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The rat IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Rat IL-1RA shares 89% and 73% a.a. sequence homology with murine and human IL-1RA.

Reference

1. Dinarello CA. 1994. FASEB J. 8:1314-25

2. Patterson D, Jones C, Hart I, et al. 1993. Genomics. 15:173-6

3. Steinkasserer A, Spurr NK, Cox S, et al. 1992. Genomics. 13:654-7

4. Perrier S, Darakhshan F, Hajduch E. 2006. FEBS Lett. 580:6289-94

5. Gabay C, Porter B, Fantuzzi G, et al. 1997. J Immunol. 159:5905-13.

Information

Gene ID	60582
Accession #	P25086
Alternate Names	IL-1RN, IRAP, IL1 Inhibitor
Source	Escherichia coli.
M.Wt	Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
AA Sequence	HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ
Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 10³ IU/mg in the presence of 50 pg/ml rRtIL-1α.
Shipping Condition	Gel pack.
Handling	Centrifuge the vial prior to opening.
Usage	For Research Use Only! Not to be used in humans.

Quality Control

Purity > 96 % by SDS-PAGE and HPLC analyses.
Endotoxin: Less than 1 EU/μg of rRtIL-1RA as determined by LAL method.

Datasheet

Recombinant Rat Interleukin-1 Receptor Antagonist Protein

Katalog-Nummer P1372-500ug

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Marke : APExBIO Technology

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Background

Information

Quality Control