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Rabbit Polyclonal Anti-Chromogranin A / CHGA (Neuroendocrine Marker)
Katalog-Nummer NB-36-00066-P1
Size : 100ug
Marke : Neo Biotech
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Telefonnummer : +1 850 650 7790
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Printable Datasheet
Chromogranin A / CHGA (Neuroendocrine Marker) Antibody []
Catalog Number Formulation Size Price
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Parathyroid stained with Chromogranin A Rabbit Polyclonal Antibody.
Formalin-fixed, paraffin-embedded human Pancreas stained with Chromogranin A Rabbit Polyclonal Antibody.
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
Product Properties & Targets
Antibody Type
Rabbit Polyclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasmic vesicle, Neuronal dense core vesicle, Secreted, Secretory vesicle
Gene Name
CHGA
Positive Control
Adrenal gland, bowel, pancreas or pheochromocytoma or parathyroid. PC12 cells.
Immunogen
Recombinant human chromogranin A protein
Alternate Names
Chromogranin-A, Pituitary secretory protein I, Beta-Granin; CGA; CHGA; Chromogranin A Parathyroid Secretory Protein 1; ER-37; Pancreastatin; Parastatin; Parathyroid Secretory Protein 1; Pituitary Secretory Protein I; SP-I; Vasostatin I or II
Functions
- Strongly inhibits glucose induced insulin release from the pancreas.
- Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).
- Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Key References
- Konecki DS et. al. J Biol Chem 1987;262:17026-30.
- Lloyd RV et. al. Am J Pathol 1988; 130:296-304.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2445752/
- https://pubmed.ncbi.nlm.nih.gov/3403545/
- https://pubmed.ncbi.nlm.nih.gov/8120054/
- https://pubmed.ncbi.nlm.nih.gov/14702039/
- https://pubmed.ncbi.nlm.nih.gov/12508121/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/3704195/
- https://pubmed.ncbi.nlm.nih.gov/2165909/
- https://pubmed.ncbi.nlm.nih.gov/2830133/
- https://pubmed.ncbi.nlm.nih.gov/1577173/
- https://pubmed.ncbi.nlm.nih.gov/12442257/
- https://pubmed.ncbi.nlm.nih.gov/7535395/
- https://pubmed.ncbi.nlm.nih.gov/9852066/
- https://pubmed.ncbi.nlm.nih.gov/10781584/
- https://pubmed.ncbi.nlm.nih.gov/14997482/
- https://pubmed.ncbi.nlm.nih.gov/15723172/
- https://pubmed.ncbi.nlm.nih.gov/16807684/
- https://pubmed.ncbi.nlm.nih.gov/18541522/
- https://pubmed.ncbi.nlm.nih.gov/19838169/
- https://pubmed.ncbi.nlm.nih.gov/20116404/
- https://pubmed.ncbi.nlm.nih.gov/21214543/
- https://pubmed.ncbi.nlm.nih.gov/23234360/
- https://pubmed.ncbi.nlm.nih.gov/24723458/
- https://pubmed.ncbi.nlm.nih.gov/24275569/
- https://pubmed.ncbi.nlm.nih.gov/14759560/
- https://pubmed.ncbi.nlm.nih.gov/14740315/
- https://pubmed.ncbi.nlm.nih.gov/15326220/
- https://pubmed.ncbi.nlm.nih.gov/17438154/
- https://pubmed.ncbi.nlm.nih.gov/17991725/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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