Rabbit IgG (H+L chain) Sheep Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF
Recommended Dilution	Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000.
Reactivities	Rabbit
Host	Sheep
Clonality	Polyclonal
Immunogen	Rabbit IgG, whole molecule
Specificity	Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer. Label: TRITC State: Lyophilized purified Ig fraction Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 2.9 moles TRITC per mole of Sheep IgG
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	2.0 mg/ml (by UV absorbance at 280 nm)
Purification	Immunoaffinity Chromatography using Rabbit IgG coupled to agarose.
Conjugation	TRITC
Storage	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.