Protein G Rabbit Polyclonal Antibody
Katalog-Nummer AP09166FC-N
Size : 20mg
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| Product Data | |
| Application | FC, IF |
|---|---|
| Application | Immunofluorescence: 1/500 - 1/2500. Flow Cytometry: 1/2000 - 1/10000. |
| Reactivity | Streptococcus |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Protein G [Streptococcus species] |
| Specificity | This antibody reacts to Protein G. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) as stabilizer and 0.01% Thimerosal as preservative Label: FITC State: Lyophilized IgG fraction Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm |
| Reconstitution Method | Restore with 2.0 ml of deionized water (or equivalent). |
| Concentration | lot specific |
| Purification | Ion exchange chromatography |
| Conjugation | FITC |
| Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Background | Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.