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Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) (PDL1/2746), RPE conjugate, 0.1mg/mL

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Katalog-Nummer BNCR2746-250

Size : 250uL

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Biotium

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Contact local distributor :

Telefonnummer : +1 850 650 7790

PD-L1 / PDCD1LG1 / CD274 / B7-H1 Monoclonal Mouse Antibody (PDL1/2746)

Product Attributes

Antibody number	#2746
Antibody reactivity (target)	B7-H1, CD274, PD-L1, PDCD1LG1
Antibody type	Primary
Host species	Mouse
Clonality	Monoclonal
Clone	PDL1/2746
Isotype	IgG2b, kappa
Molecular weight	37-50 kDa
Synonyms	B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1
Human gene symbol	CD274
Entrez gene ID	29126
SwissProt	Q9NZQ7
Unigene	521989
Immunogen	Recombinant fragment of human CD274 protein (around aa 39-191) (exact sequence is proprietary)
Antibody target cellular localization	Secreted (extracellular)
Verified antibody applications	Flow (surface) (verified), IF (verified), IHC (FFPE) (verified), WB (verified)
Species reactivity	Human, Mouse
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, Western blotting 1-2 ug/mL, Flow Cytometry 1-2 ug/million cells in 0.1 mL, ELISA For coating, order Ab without BSA, Optimal dilution for a specific application should be determined by user
Positive control	Heart, Placenta, Spleen or Tonsil tissue. RAW, HEK293 or HepG2 cell lysates. Jurkat Cells.
Shipping condition	Room temperature
Storage Conditions	Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life	Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status	For research use only (RUO)
Antibody/conjugate formulation	Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Validated in protein array	Monospecific
Antibody research areas	Apoptosis, Cancer, Cancer immunotherapy/immune checkpoint
Product origin	Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Technology

CF® Dyes

Considerations for Immunofluorescence Staining

Primary & Secondary Antibody Conjugates

Product Description

PD-L1 (also known as Programmed Death Ligand 1, CD274, and B7-H1) is one of two cell surface ligands that engage the negative regulatory T cell surface receptor PD-1. PD-L1 is a Type I transmembrane protein expressed on the plasma membrane of cells, such as activated macrophages and some tumor cells. Because binding of PD-1 by PD-L1 has a suppressive effect on the immune system, inhibition of the PD-1/PD-L1 pathway is a major strategy of immune-oncology therapies. PD-L1 is upregulated in certain cancers, and blocking PD-1 or PD-L1 can inhibit cancer growth.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@biotrend.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 2746, Anti-B7-H1|CD274|PD-L1|PDCD1LG1 (PDL1/2746)

Antibody # prefix	Conjugation	Ex/Em (nm)	Laser line	Detection channel	Dye Features
BNC04	CF®405S	404/431	405	DAPI (microscopy), AF405	CF®405S Features
BNC88	CF®488A	490/515	488	GFP, FITC	CF®488A Features
BNC68	CF®568	562/583	532, 561	RFP, TRITC	CF®568 Features
BNC94	CF®594	593/614	561	Texas Red®	CF®594 Features
BNC40	CF®640R	642/662	633-640	Cy®5	CF®640R Features
BNC47	CF®647	650/665	633-640	Cy®5	CF®647 Features
BNC74	CF®740	742/767	633-685	775/50	CF®740 Features
BNCB	Biotin	N/A	N/A	N/A
BNUB	Purified	N/A	N/A	N/A
BNUM	Purified, BSA-free	N/A	N/A	N/A

Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

Note: Listed references are for this antibody clone sold by Biotium and other suppliers.