p53 (Tumor Protein p53, Tumor Suppressor p53, TP53, TRP53, Cellular Tumor Antigen p53, Phosphoprotein p53, Antigen NY-CO-13, LFS1) (HRP)

p53 is a 53kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.||Applications:|Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.||Recommended Dilution:|Immunohistochemistry: Frozen.|Immunohistochemistry (Paraffin): 1:1000|Western Blot: 1:1000|Note: Requires antigen retrieval; sodium citrate buffer pH 6.0 is recommended . Recommended positive control tissue: Colon or breast carcanoma.|Optimal dilutions to be determined by the researcher.||Recommended Secondary Reagents: |I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo|I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo||Recommended Negative Controls (IHC): |I1904-83P1: IgG2a Negative Control||Hybridoma: |P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.