Neutral Red Cell Proliferation Assay Kit

Neutral Red Cell Proliferation Assay Kit is a kit that can quickly detect cell proliferation or toxicity. The uptake of neutral red by cells is determined by the ability of cells to maintain a pH gradient, which is dependent on ATP production. Under physiological pH conditions, neutral red can enter the cell membrane by passive diffusion and accumulate in lysosomes. When the number of cells increases, the intake of neutral red also increases. Conversely, when cells are damaged, their ability to ingest neutral red is diminished. By measuring the amount of neutral red ingested by the cells, the proliferation or toxicity of the cells can be assessed.

In addition, neutral red can also be used as a pH indicator, and its color will change with the pH value. Neutral red appears red in acidic environments, and gradually changes color from red to yellow when the pH rises from 6.8 to 8.0.

Figure 1: Standard curve for this kit