Neurofilament NF-H

Katalog-Nummer NB-26-01993

Size : 100ul

Marke : Neo Biotech

Weitere Informationen anfordern

Contact local distributor :


Telefonnummer : +1 850 650 7790

Product Details
Applications: ICC, WB, IHC, IF
Type: Chicken IgY
Dilutions: Immunofluorescence: 1:1,000-5,000; Immunohistochemistry: 1:1,000-5,000; Western Blot: 1:10,000-25,000
Immunogen: Native NF-H purified from bovine spinal cord
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez:
UniProt:
Immunofluorescence

Immunofluorescence analysis of mouse cerebellum section stained with goat pAb to NF-H, GT22109, dilution 1:3,000 in red, and costained with mouse mAb to myelin basic protein (MBP) in green. The NF-H antibody labels axons of basket and Purkinje cells and others. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons in the granular layer at center and the white matter at bottom left.

Immunofluorescence

Immunofluorescence analysis of mouse cerebellum section stained with goat pAb to NF-H, GT22109, dilution 1:3,000 in red, and costained with mouse mAb to myelin basic protein (MBP) in green. The NF-H antibody labels axons of basket and Purkinje cells and others. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons in the granular layer at center and the white matter at bottom left.

Western Blot

Western blot analysis of tissue lysates from different species using goat pAb to NF-H, GT22109, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, [5] cow spinal cord, [6] pig hippocampus and [7] pig spinal cord. Strong band at about 220kDa corresponds to the major phospho-NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.

Western Blot

Western blot analysis of tissue lysates from different species using goat pAb to NF-H, GT22109, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, [5] cow spinal cord, [6] pig hippocampus and [7] pig spinal cord. Strong band at about 220kDa corresponds to the major phospho-NF-H subunit. Smaller proteolytic fragments of NF-H are also detected in some preparations.