N-Cadherin / Cadherin-2 / CD325 (NCAD) (13A9), 0.2mg/mL
Katalog-Nummer BNUB1425-500
Size : 500uL
Marke : Biotium
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Antibody number | #1425 |
|---|---|
| Antibody reactivity (target) | Cadherin-2, CD325, N-Cadherin |
| Antibody type | Primary |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone | 13A9 |
| Isotype | IgG1, kappa |
| Molecular weight | 130-140 kDa |
| Synonyms | Cadherin-2 N cadherin neuronal; Cadherin-2 type 1; Cadherin-2; Calcium dependent adhesion protein neuronal; CD325; CDH2; CDHN; CDw325; N-Cadherin; NCAD |
| Human gene symbol | CDH2 |
| Entrez gene ID | 1000 |
| SwissProt | P19022 |
| Unigene | 464829 |
| Immunogen | Recombinant human N-cadherin cytoplasmic domain (exact sequence is proprietary) |
| Antibody target cellular localization | Plasma membrane |
| Species reactivity | Human, Mouse |
| Expected antibody applications | IHC (FFPE) (published for clone), IP (published for clone), WB (published for clone) |
| Antibody application notes | Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user |
| Positive control | HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex |
| Shipping condition | Room temperature |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Regulatory status | For research use only (RUO) |
| Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
| Antibody research areas | Cancer, Cell adhesion, Developmental biology |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
| Tumor expression | Metastatic cells |
Product Description
This antibody recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the
CF® Dye Brochure
for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.info@biotrend.com
to inquire about stock status and lead times before placing your order.Catalog number key for antibody number 1425, Anti-Cadherin-2 (13A9)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |
References
Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Biol Chem (1995) 270(34): 20201-20206. (IP; WB)
- J Cell Biol Volume (1996) 135(6 Pt 1): 1643-1654. (IP; WB; IF)
- J Histol Histopathol (2014) 1:4. http://dx.doi.org/10.7243/2055-091X-1-4 (IHC, FFPE)