Western blot analysis of over-expressed His-tagged protein in 293T cell lysate.

Immunofluorescent analysis of His-tag staining in 293T cells transfected with a His-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation of His-tagged protein from HEK293T cells transfected with vector overexpressing His tag, using Anti-His-tag Antibody.