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Mouse Monoclonal Anti-CD44v4 (Marker of Tumor Metastasis) [Clone CD44v4/1219]
Katalog-Nummer NB-36-01000-P0
Size : 20ug
Marke : Neo Biotech
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Printable Datasheet
CD44v4 (Marker of Tumor Metastasis) Antibody [CD44v4/1219]
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Formalin-fixed, paraffin-embedded human Cervical Squamous Cell Carcinoma stained with CD44v4 Mouse Monoclonal Antibody (CD44v4/1219)
Formalin-fixed, paraffin-embedded human Tongue Squamous Cell Carcinoma stained with CD44v4 Mouse Monoclonal Antibody (CD44v4/1219)
SDS-PAGE Analysis of Purified CD44v4 Mouse Monoclonal Antibody (CD44v4/1219). Confirmation of Purity and Integrity of Antibody.
Applications & Dilutions
Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Summary
This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Articular Cartilage Extracellular Matrix, Cytokine Signaling, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation, Neural Stem Cells
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell membrane, Cell projection, Microvillus
Gene Name
CD44
Positive Control
Cervix or Tongue Squamous Cell Carcinoma.
Immunogen
Recombinant fragment corresponding to the v4 domain of human CD44
Alternate Names
CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, LHR; BA-1; chondroitin sulfate proteoglycan 8 (CSPG8); Epican; Extracellular Matrix Receptor III (ECM III); GP90 Lymphocyte Homing Adhesion Receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan Sulfate Proteoglycan; Hermes Antigen; HAS; HUTCH I; Hyaluronate Receptor; Indian blood group; Inlu Related p80 Glycoprotein; Ly 24; MDU2; MDU3; MIC4; MUTCH I; Phagocytic Glycoprotein 1 (PGP-1)
Functions
- Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).
Key References
- Thanakit V, Sampatanukul P, Ruangvejvorachai P, Keelawat S. The association of co-expression of CD44v4/MMP-9 with different nodal status in high-grade breast carcinoma patients. J Med Assoc Thai. 2005 Sep;88 Suppl 4:S30-5.
- H. Imam, B. Eriksson K. Oberg. Expression of CD44 variant isoforms and association to the benign form of endocrine pancreatic tumours. Annals of Oncology 11: 295-300, 2000.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2466575/
- https://pubmed.ncbi.nlm.nih.gov/1840487/
- https://pubmed.ncbi.nlm.nih.gov/1991450/
- https://pubmed.ncbi.nlm.nih.gov/2056274/
- https://pubmed.ncbi.nlm.nih.gov/1281868/
- https://pubmed.ncbi.nlm.nih.gov/1465456/
- https://pubmed.ncbi.nlm.nih.gov/7508842/
- https://pubmed.ncbi.nlm.nih.gov/8352881/
- https://pubmed.ncbi.nlm.nih.gov/10933060/
- https://pubmed.ncbi.nlm.nih.gov/17974005/
- https://pubmed.ncbi.nlm.nih.gov/16554811/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/1922057/
- https://pubmed.ncbi.nlm.nih.gov/2466576/
- https://pubmed.ncbi.nlm.nih.gov/7527301/
- https://pubmed.ncbi.nlm.nih.gov/7508992/
- https://pubmed.ncbi.nlm.nih.gov/2007624/
- https://pubmed.ncbi.nlm.nih.gov/8148709/
- https://pubmed.ncbi.nlm.nih.gov/1717145/
- https://pubmed.ncbi.nlm.nih.gov/7528188/
- https://pubmed.ncbi.nlm.nih.gov/12511867/
- https://pubmed.ncbi.nlm.nih.gov/9580567/
- https://pubmed.ncbi.nlm.nih.gov/12032545/
- https://pubmed.ncbi.nlm.nih.gov/12883358/
- https://pubmed.ncbi.nlm.nih.gov/15123640/
- https://pubmed.ncbi.nlm.nih.gov/16335952/
- https://pubmed.ncbi.nlm.nih.gov/17081983/
- https://pubmed.ncbi.nlm.nih.gov/16541107/
- https://pubmed.ncbi.nlm.nih.gov/19367720/
- https://pubmed.ncbi.nlm.nih.gov/18691976/
- https://pubmed.ncbi.nlm.nih.gov/18669648/
- https://pubmed.ncbi.nlm.nih.gov/19159218/
- https://pubmed.ncbi.nlm.nih.gov/19381274/
- https://pubmed.ncbi.nlm.nih.gov/19690332/
- https://pubmed.ncbi.nlm.nih.gov/19703720/
- https://pubmed.ncbi.nlm.nih.gov/18757307/
- https://pubmed.ncbi.nlm.nih.gov/20962267/
- https://pubmed.ncbi.nlm.nih.gov/20068231/
- https://pubmed.ncbi.nlm.nih.gov/21269460/
- https://pubmed.ncbi.nlm.nih.gov/21406692/
- https://pubmed.ncbi.nlm.nih.gov/22171320/
- https://pubmed.ncbi.nlm.nih.gov/22726066/
- https://pubmed.ncbi.nlm.nih.gov/23589287/
- https://pubmed.ncbi.nlm.nih.gov/23186163/
- https://pubmed.ncbi.nlm.nih.gov/24275569/
- https://pubmed.ncbi.nlm.nih.gov/25944712/
- https://pubmed.ncbi.nlm.nih.gov/29777959/
- https://pubmed.ncbi.nlm.nih.gov/14992719/
- https://pubmed.ncbi.nlm.nih.gov/17085435/
- https://pubmed.ncbi.nlm.nih.gov/25195884/
- https://pubmed.ncbi.nlm.nih.gov/8636151/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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