MIDN (Midnolin, Midbrain Nucleolar Protein)

Expression of the midnolin gene is developmentally regulated: it is strongly expressed at the mesencephalon (midbrain) of the embryo in day 12.5 (E12.5) mice. The midnolin encodes a protein of 508aa (aa), which contains a Ubiquitin-like domain. The intracellular distribution of the midnolin was studied by using midnolin-green fluorescent protein (GFP) fusion proteins. Midnolin was found to be localized in the nucleus and nucleolus, but not in the cytoplasm. The nucleolar localization signal was determined to be a 28aa peptide located at the C-terminal region of the midnolin. May be involved in regulation of genes related to neurogenesis in the nucleolus.||Applications: |Suitable for use in ELISA, Western Blot. Other applications not tested.||Recommended Dilution:|ELISA: 1:1000-1:5000|Western Blot: 1:500-1:2000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.