MHC Class I (monomorphic) mouse monoclonal antibody, clone 2G5, FITC
Specifications
Product Data
Clone Name
2G5
Application
FC
Recommended Dilution
Flow Cytometry: Use 10 µl of Neat-1/5 diluted antibody to label 10e6 cells in 100 µl.
Reactivity
Bovine, Guinea Pig, Hamster, Human, Mouse, Porcine, Rat
Antibody Host
Mouse
Isotype
IgG2b
Clonality
Monoclonal
Immunogen
Purified H-2Kb and H-2Db MHC-I molecules. Spleen cells from immunised C1D mice were fused with cells of the X63 myeloma cell line.
Specificity
This antibody recognises a monomorphic epitope on the MHC class I antigen. The epitope recognised by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some Canine samples suggesting that this antibody may recognise a polymorphic epitope of Canine MHC class 1.
Buffer
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration
lot specific
Purification
Affinity chromatography on Protein G
Conjugation
FITC
Storage
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Stability
Shelf life: one year from despatch.
Shipping
Blue Ice
Background
MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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