Melanin Concentrating Hormone, Human (MCH) Control Peptide

Control Peptide for M2867-70C (affinity purified antibody) or M2867-70D (antiserum).||Melanin-concentrating hormone (MCH) is a cyclic neuropeptide (human/rat 19aa) that regulates a variety of functions in mammalian brain, in particular stimulates feeding behavior. MCH is synthesized mainly in the lateral hypothalamus and zona incerta. Its level is unregulated in the hypothalamus of genetically obese mice. Mice lacking MCH eat less and are leaner. MCH is thought to influence feeding and energy balance by acting downstream of leptin and the melanocortin system. Molecular mechanisms of these diverse actions of MCH remain poorly understood. An orphan G-protein coupled receptor (SLC-1, GPR24) has been identified as the receptor of MCH. MCH receptor (MCHR; human 402aa, rat 353aa) is predicted to contain 7 transmembrane domains, a feature typical of G-protein coupled receptors. It is primarily expressed in the ventromedial and dorsomedial nuclei of the hypothalamus. Moderate levels of MCHR are also found in the eye and skeletal muscle, tongue and pituitary. MCHR binds MCH with sub-nanomolar affinity. It is stimulated by MCH to mobilize intracellular Ca2+ and reduce forskolin-elevated cAMP levels.||Applications:|Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.||Recommended Dilution:|ELISA: 50-100ng of control peptide/well|Antibody Blocking: 5-10ug per 1ug M2867-70C (affinity purified antibody) or per 1ul M2867-70D (antiserum).||Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.