Mast Cell Tryptase (BSA & Azide Free) (HRP)

Katalog-Nummer M2415-01X-HRP-100ul

Size : 100ul

Marke : US Biological

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M2415-01X-HRP Mast Cell Tryptase (BSA & Azide Free) (HRP)

Clone Type
Polyclonal
Host
mouse
Source
human
Isotype
IgG1
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Ca Fe Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C

Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. A substantial quantity of tryptase is estimated to be found in mast cells of skin and lung and suggestes this enzyme plays a major role in mast cell mediated events. In vitro studies indicate tryptase can cleave C3 to form C3a anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin and activate latent collagenase. Human mast cell tryptase has considerable potential as a marker for mast cell activation as well as being a mediator of inflammation. ||Applications: |Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilutions: |Immunohistochemistry (Formalin/paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 minutes followed by cooling at RT for 20 minutes. |Optimal dilutions to be determined by the researcher.||Recommended Positive Control: |Tonsil||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 1.N.5|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Human mast cell tryptase isolated from purified human lung tissues|Cellular Localization: Cytoplasmic|Specificity: Recognizes human Mast Cell Tryptase. Reacts with Mast Cell Tryptase, both a and b isoforms. Species Crossreactivity: monkey, canine and feline.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human mast cell tryptase isolated from purified human lung tissues|Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Mast Cell Tryptase. Reacts with Mast Cell Tryptase, both a and b isoforms. Species Crossreactivity: monkey, canine and feline.
References
1. Walls, A.F., et al., (1990) Clin. Exp. Allergy 20: 581-590. 2. Walls, A.F., et al., (1990) J. Pathol. 162: 119-126. 3. Goldsmith, P., et al., (1990) Digestive Diseases and Sciences 35(11): 1,409-1,413.