Magic Red™ Cathepsin B BioAssay™ Kit

Sample Type:|Cell culture||Reagent Name: |MR-RR2||Target:|Cathepsin-B||Excitation/Emission:|592nm/628nm||Method of Analysis:|Fluorescence Plate Reader, Fluorescence Microscope||Intended Use:|Quantitate and monitor intracellular cathepsin-B activity over time in vitro. The Magic Red substrate in this BioAssay™ fluoresces red upon cleavage by active cathepsin enzymes. Analyze the fluorescent signal using fluorescence microscopy or a fluorescence plate reader.||Test Principle:|Elevated cathepsin enzyme activity in serum or the extracellular matrix can signify a number of pathological conditions. Magic Red BioAssay™ kits are used by researchers seeking to quantitate and monitor cathepsin activity in cultured cells and tissues. This BioAssay™ kit detects cathepsin-B activity.||To use Magic Red, add the substrate directly to the cell culture media, incubate, and analyze. Because it is cell permeant, it easily penetrates the cell membrane and the membranes of the internal cellular organelles – no lysis or permeabilization steps are required. If cathepsin enzymes are active, the Magic Red substrate is cleaved and the cresyl violet fluorophore will become fluorescent upon excitation. As enzyme activity progresses and more Magic Red substrate is cleaved, the signal will intensify, allowing researchers to watch the color develop over time. Samples can be analyzed by fluorescence microscopy or with a fluorescence plate reader. Hoechst 33342 and Acridine Orange are included in the BioAssay™ kit to detect nuclear morphology and lysosomal organelle structure, respectively.||Kit Components:|25 tests:|Magic Red Substrate (MR-RR2), 1 25 Test Vial|Hoechst 33342, 1x1mL|Acridine Orange, 1x0.5mL||100 tests:|Magic Red Substrate (MR-RR2), 1 100 Test Vial|Hoechst 33342, 1x1mL|Acridine Orange, 1x0.5mL||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.