Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (Biotin)

The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.||Applications:|Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Membrane permeabilization is required for this application.|Immunohistochemistry (Frozen and Paraffin): Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment.|Optimal working dilutions to be determined by researcher.||Recommended Positive Control:|Human spleen||Recommended Negative Control: |I1904-78R: IgG1: Murine Negative Control||Recommended Secondary Antibodies:|I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo|I1904-66C: IgG, F(ab’)2 X-adsorbed (HRP) Pab Rb x Mo|I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo||Hybridoma: |NS1 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.