IL-3 (Burst promoting activity, Colony stimulating factor, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL 3, Interleukin 3) (Biotin)

The pleiotropic IL3 (Interleukin 3) is a 15kD cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.||Applications:|Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|ELISA: 1:20,000-1:100,000|Western Blot: 1:2000-1:10,000|Immunohistochemistry: 1:1000-1:5000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.