ICAM-1 BioAssay™ ELISA Kit, Rat

Sandwich ELISA quantitative detection kit for Rat ICAM-1 in cell culture supernates, serum and plasma (heparin, EDTA).||Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1).2 ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1 The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. ||Assay Range:|31.2pg/ml-2000pg/ml||Sensitivity:|< 2 pg/ml||Crossreactivity:|There is no detectable cross-reactivity with other relevant proteins.||Uniprot ID:|P05362||Entrez Gene ID:|3383||Kit Components:|96-well plate pre-coated with anti-ICAM-1, Rat|Lyophilized recombinant ICAM-1, Rat standard, 10ng/tube, 2Ea|USB Cat No Kit Component|143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)|143697 Sample diluent buffer, 30ml|143698 Antibody diluent buffer, 12ml|143699 ABC diluent buffer, 12ml|143700 TMB color developing agent, 10ml|143701 TMB stop solution, 10ml||Storage and Handling:|Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.