HyperScribe™ All in One mRNA Synthesis Kit (ARCA, T7, poly(A))
Katalog-Nummer K1063-25
Size : 25rxns
Marke : APExBIO Technology
Contact local distributor :
Telefonnummer : +1 850 650 7790
HyperScribe™ All in One mRNA Synthesis Kit (ARCA, T7, poly(A))
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
- 1. Ning Luan, Han Cao, et al. "Comparison of Immune Responses between Inactivated and mRNA SARS-CoV-2 Vaccines Used for a Booster Dose in Mice." Viruses. 2023 Jun 11;15(6):1351. PMID: 37376650
HyperScribe™ All in One mRNA Synthesis Kit (ARCA, T7, poly(A)) is designed for quick production of ARCA capped and poly(A) tailed mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA, Catalog No. B8175) using T7 RNA Polymerase. The capping of ARCA ensures high translation efficacy. After a brief DNase I treatment to remove the template DNA, capped mRNA is poly(A) tailed with Poly(A) Polymerase. Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation.
RNA synthesized using this kit has many applications in biological experiments, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.
The kit contains sufficient reagents to carry out 25 reactions, 20 μL each time. Up to 50 μg of RNA can be generated with 1 μg of control template per standard reaction.
An upgraded version of this product is available with higher yield (~100 μg), please check out K1406 HyperScribe™ Co-transcription mRNA Synthesis Kit Plus (ARCA, T7) for more details. (Note: it does not include the reagents for the poly A tailing, please incorporate the poly A sequence in your plasmid or primer).(1) Capped RNA Synthesis with ARCA | |
| Components | 25 rxns |
| T7 RNA Polymerase Mix | 50 μL |
| 10X Reaction Buffer | 50 μL |
| ATP (20 mM) | 50 μL |
| UTP (20 mM) | 50 μL |
| CTP (20 mM) | 50 μL |
| GTP (20 mM) | 37.5 μL |
| ARCA (60 mM) | 50 μL |
| Control Template (0.5 μg/μL) | 5 μL |
| RNase-free H2O | 0.5 mL |
Store all kit components at -20°C. | |
(2) Tailing Reaction | |
| Components | 25 rxns |
| E-PAP (2 units/μL) | 100 μL |
| 5X E-PAP Buffer | 600 μL |
| ATP Solution (100 mM) | 100 μL |
| 25 mM MnCl2 | 250 μL |
| Nuclease - free Water | 1 mL x 2 |
Store all kit components at -20°C. | |
- K1064 HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, ψUTP, T7, poly(A))
- K1065 HyperScribe™ All in One mRNA Synthesis Kit Plus 2 (ARCA, 5-moUTP, T7, poly(A))